Anyone here have experience extracting enzymes/proteins from filamentous fungi? Hi everyone. I really, really need the help of this community. I'm a student in my first year of a Master's degree, and my project involves identifying filamentous fungi that are capable of producing a certain class of enzymes capable of degrading a certain polymer. I cannot be more specific due to proprietary information, sorry. The fungi I am screening are environmental isolates, and as such I do not know their identity beyond genus level. The database includes Trichoderma, Cladosporium, Penicillium and various unknown isolates. Currently I am cultivating the fungi in minimal media with the polymer present as an inducer. Every possible control is also included, please ask if you would like more detail on these. We are running turbidity-based enzyme assays on the supernatant present after 2 and 3 weeks of cultivation, but getting no activity.…