Nature, Published online: 18 March 2026; doi:10.1038/d41586-026-00730-6

A genome-editing approach called INSTALL combines single-stranded DNA molecules that evade immune detection with a short double-stranded region that allows recombinase enzymes to insert a DNA sequence into mammalian genomes. INSTALL enables the insertion of large DNA sequences into the genomes of human and mouse cells without causing toxic immune responses.