A buddy of mine has put together an online tool to help you design CRISPR reagents for easy diagnosis. Basically you plug in the DNA sequence of the gRNA recognition region and it works out which restriction sites can be destroyed and introduced by all the potential wobbles. This way you have a positive and negative restriction screen for easy testing of clones. I had the idea but he threw together the code. It is entirely free. submitted by /u/Novel-Structure-2359 [link] [comments]