Article Published: 27 April 2026 Nature ( 2026 ) Cite this article We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply. Subjects Abstract Telomere-to-telomere (T2T) phased assemblies are emerging as a benchmark for reference-quality genomes 1,17 , though they remain technically and financially demanding, particularly at scale. Generating such assemblies for diploid and polyploid genomes typically involves combining high-accuracy long reads, such as PacBio HiFi 16 or the now-deprecated ONT Duplex 2 reads, with ultra-long ONT Simplex reads. Using multiple platforms or methods increases the cost and the required amount of genomic DNA.…