Hi everyone, I'd like to predict a protein complex involving a target protein and polyubiquitin chains with covalent linkage. However, our lab does not currently have access to HPC resources or local servers capable of running AlphaFold3. I tried using the Boltz-2 and Chai-1 webservers, but unfortunately my target protein exceeds their sequence length limitations. Are there any other web-based tools or servers that could handle this kind of prediction? Or is using cloud GPU services (e.g. AWS, Google Cloud, etc.) basically the only realistic option for large AF3-like complex predictions? Any suggestions or experiences would be greatly appreciated. Thanks! submitted by /u/Latter-Election7109 [link] [comments]