📰00at my the end of my rope trying to purify this protein.Reddit r/Biochemistry·u/Danktank452·about 1 month ago#1Mq4tnwm#protein#column#imidazole#using#purify#article+2 more🧰Tag tools✨Add tagSo I recently started a postdoc and have been tasked with purifying a microsomal P450 enzyme. I did purification of his tagged heme proteins during my PhD and ever had any trouble. This P450 will not stick to the column no matter what I do.… Read more15s0Read later0Read More